Discussion on Treatment of Recurrent Genital Herpes with Zhulingtang： Based on Thought of "Lower Energizer Resembling Drainage" / 中国实验方剂学杂志

Recurrent genital herpes is a sexually transmitted disease induced by herpes simplex virus（HSV）， mainly manifested as erythema， papules， vesicles， erosion， and exudation on the skin and mucosal membranes of the genital and anal area， accompanied by burning pain， or tingling. Factors leading to its recurrence are overworking， fever， alcohol consumption， infection， sexual activity， etc. At least 1 in 10 of the 15-49-year-old population are infected with herpes simplex virus-2. There is currently no available method to completely remove the herpes simplex virus and prevent its recurrence， and recurrence seriously affects the quality of life and psychological health of patients. According to the theory of traditional Chinese medicine （TCM）， the recurrence of genital herpes is mostly related to wind， dampness， heat， yin deficiency of liver and kidney， internal injuries due to emotional disorder， and dietary preference. TCM has advantages in reducing its recurrence rate. The lower energizer is closely related to skin diseases， and "lower energizer resembles drainage". Thus， the lower energizer is closely related to water and damp metabolism. Recurrent genital herpes occurs in the lower energizer and belongs to lower energizer diseases， and the fluid metabolism and qi movement in lower energizer are closely related to the occurrence of the disease. Based on thought of "lower energizer resembling drainage" and the clinical characteristics of recurrent genital herpes， this paper considered malfunction of lower energizer as its pathogenesis. Thus， smoothing the lower energizer according to the prevailing circumstances is particularly important and the treatment principle should be dispersing the pathogen from lower energizer without damaging yin. Zhulingtang （Treatise on Cold Damage and Miscellaneous Diseases） cures the difficulty in urination and thirst with desire to drink by smoothing the lower energizer. Thus， Zhulingtang was selected to treat recurrent genital herpes. This study is expected to provide a new idea for its clinical diagnosis and treatment.

Evaluation of Compatibility Proportion and Antidepressant Mechanism of Bupleuri Radix-Scutellariae Radix Based on Uniform Design / 中国实验方剂学杂志

Objective:To optimize proportion of couplet medicine of Bupleuri Radix and Scutellariae Radix in the treatment of anti-depression, and to explore the possible antidepressant mechanism of this couplet medicine. Method:The dosages of Bupleuri Radix and Scutellariae Radix in the 2015 edition of Chinese Pharmacopoeia were taken.According to U7(72) uniform design table, Bupleuri Radix and Scutellariae Radix were carried out into 7 groups.The chronic unpredictable mild depression model mice were induced by intragastric administration of decoction of this couplet medicine and the antidepressant effect was observed by the behavior tests, which included sucrose preference test, tail suspension test, forced swimming test and open field test(crossing scores).The regression equations were established by selecting the effective indexes.The experiments of retest were taken to check the results and the possible antidepressant mechanism was primarily investigated by measuring the phosphorylation level of cyclic adenosine monophosphate(cAMP)-response element binding protein(CREB) and expression of brain-derived neurotrophic factor(BDNF). Result:Compared with the blank group, sucrose preference rate of the model group was significantly decreased(PPPPPPPPPPPPPPConclusion:Compatibility of Bupleuri Radix and Scutellariae Radix can ameliorate depressive-like behavior of model mice, and the best antidepressant compatibility proportion of Bupleuri Radix and Scutellariae Radix is 1:1, the optimal amounts of them are about 5 g.The antidepressant effect may relate to promoting phosphorylation level of CREB and the expression of BDNF in the hippocampus.

Effect of P53 Expression on Prognosis of Patients with Double Expressor Lymphoma / 中国实验血液学杂志

OBJECTIVE@#To investigate the effect of P53 expression on prognosis of patients with double expressor lymphoma(DEL) and the interaction between the expression of MYC, BCL2 and P53 in diffuse large B-cell lymphoma(DLBCL).@*METHODS@#Eighty-eight patients with newly diagnosed DLBCL from 1st September 2012 to 31th May 2018 in Shanxi Dayi Hospital affiliated to Shanxi Medical University were selected. The expressions of MYC、BCL2、P53、CD10、BCL6、MUM and Ki-67 were tested by immunohistochemistry method. The overall survival of patients was analyzed by the Kaplan-Meier method and compared by the log-rank test. The prognostic effect of MYC, BCL2 and P53 expression was analyzed by univariate and multivariate analysis.@*RESULTS@#Compared with patients without P53 expression, the patients with P53 expression had higher LDH level, higher NCCN-IPI scores, lower response to chemotherapy，poorer overall survival(OS) and a higher rate of death(P0.05). Among lymphoma patients with MYC/P53, MYC/BCL2 and BCL2/P53 co-expression, the patients with MYC/P53 co-expression had the worse OS (3 year OS rate：31.6%), followed by the subgroup of patients with MYC/BCL2/P53(3 year OS rate：46.2%), patients with MYC/BCL2/P53 expression(3 year OS rate： 636%) showed a longer OS compared with the other two subgroups（P<0.05）. Multivariate analysis demonstrated that P53 expression and NCCN-IPI were independent prognostic factors in this patient cohort.@*CONCLUSION@#P53 and MYC expressions have a synergistically negative prognostic effect in DLBCL patients. P53 expression augments the negative prognostic effect of MYC/BCL2 double expression. Patients with MYC/P53 co-expression have a worse prognosis in comparison with the patients with MYC/BCL2 double expression.

Application of asthma predictive index-based group therapy in wheezing children under 5 years of age / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To study the application value of asthma predictive index (API)-based group therapy in wheezing children under 5 years of age.</p><p><b>METHODS</b>A total of 239 wheezing children under 5 years of age were divided into API-positive (n=126) and API-negative groups (n=113). Each group was randomly assigned to inhaled corticosteroids (ICS) subgroup and montelukast sodium (leukotriene receptor antagonist, LTRA) subgroup. The ICS and LTRA subgroups received the same drug therapy at the same dosage within the first four weeks of treatment. In the stable period of disease, the ICS subgroup only received aerosol inhalation of budesonide suspension, while the LTRA group was orally given montelukast sodium only. Asthma symptom scores were assessed and recorded at different time points.</p><p><b>RESULTS</b>In the first four weeks of treatment, ICS and LTRA were effective both in the API-positive and API-negative groups; the two groups showed significant improvements in asthma symptom scores, and the asthma symptom score showed no significant difference between the ICS and LTRA subgroups of each group. After 24 weeks of treatment, the two therapies were still effective; in the API-positive group, the LTRA subgroup had a better treatment outcome than the ICS subgroup, but there was no significant difference in treatment outcome between the LTRA and ICS subgroups of the API-negative group.</p><p><b>CONCLUSIONS</b>For wheezing children under 5 years of age, therapeutic strategies can be chosen based on API in the stable period of disease, so as to better control wheezing.</p>

Changes of natural killer T cells levels in peripheral blood of children with asthma and clinical significance / 中华实用儿科临床杂志

Objective To study the changes of natural killer T cells (NKT cells) in peripheral blood and the ability of NKT cells to proliferate in response to alpha-galactosyl ceramide (α-Galcer) in children with asthma.Methods Fifty-two asthmatic children who were diagnosed by Pediatric Clinic of Renji Hospital Affiliated to Shanghai Jiaotong University Medical College from Feb.2009 to Mar.2012 were selected as asthmatic group (26 cases in intermittent group,14 cases in mild persistent group and 12 cases in moderate persistent group).Twenty healthy children were selected as healthy control group.Peripheral blood mononuclear cells were collected by way of density gradient centrifugation method from blood samples.The ratio of peripheral blood NKT and CD4 + NKT cells were assessed by immunolluorescence and flow cytometry assays.The relationships between the NKT cells number,CD4 + NKT cells and atopic indexes such as the total IgE,eosinophil cationic protein (ECP) and the severity of asthma were investigated.The levels of IFN-γ,IL-4,IL-10 in peripheral blood were measured by enzyme-linked immunosorbent assay after proliferate in response to α-Galcer.The data were analyzed by using statistics software.Results Compared with healthy control group,the ratio of peripheral blood NKT and CD4 + NKT cells in asthmatic patients were significantly decreased (all P ＜0.01) ; There were no significant differences in asthmatic patients among the subgroups in terms of severity (P ＞ 0.05).There was no significant correlation between the NKT cells,CD4 + NKT cells and atopic indexes such as the total IgE and ECP(all P ＞ 0.05).The level of IL-4 in serum secreted by NKT cells in asthmatic group was significantly higher than that of healthy control group (P ＜0.01) ;The level of IL-10 in serum secreted by NKT cells in asthmatic group was decreased significantly (P ＜0.01) ;There was no significant difference in IFN-γ secreted by NKT cells between the asthmatic group and healthy control group (P ＞ 0.05).Conclusion The pathogenesis of asthma may be related to the ratio and dysfunction of NKT cells and CD4 + NKT cells.

Genotypic and clinical features of spinal muscular atrophy type 3 / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To explore the genotypic and clinical features and laboratory examinations of spinal muscular atrophy type 3 (SMA III).</p><p><b>METHODS</b>Results of genetic testing and laboratory exams of 18 SMA III patients were collected and analyzed.</p><p><b>RESULTS</b>The average age of onset of patients was 6.1 years, with the course of disease lasting from 13 months to 28 years. All patients became symptomatic with lower extremity muscle weakness. The symptoms gradually aggregated, with proximal lower limb muscle becoming atrophic and proximal upper limb muscle becoming weak. Genetic testing indicated that all subjects possessed homozygous deletions of SMN1 gene. Electromyography (EMG) of 15 subjects indicated neurogenic damage. Whilst younger patients had normal level of creatine kinase (CK), elder patients had higher level of CK, though no linear correlation was found.</p><p><b>CONCLUSION</b>Full understanding of Clinical, especially the growth features of SMA III, in combination with genetic testing, can facilitate diagnosis and early intervention of the disease.</p>

Effect of Brain Derived Neurotrophic Factor on the Birth Weight and the Brain Weight of Newborn Rats with Intrauterine Growth Retardation / 实用儿科临床杂志

Objective To explore the effect of brain derived neurotrophic factor(BDNF) on the birth weight and the brain weight of newborn rats with intrauterine growth retardation(IUGR).Methods Female and male Sprague-Dawley rats were mated together and the vaginal plug formation after mating was for pregnancy diagnosis.The day when the plug was observed was considered day 1 of gestation.Then,the pregnant rats were divided into IUGR group and sham surgery group.On day 17 of gestation,the uterine arteries of pregnant rats in IUGR group were clamped for 30 min by blood vessel forceps after abdominal cavity with clamping blood uessels was opened.The pegnant rats in sham surgery group only had abdominal cavity opened without clamping blood vessels.Then the pegnant rats in IUGR group received 9 g/L saline(IUGR group) or BDNF 1 ?g injected in the rat through caudal veins for 5 days after surgery(BDNF intervention group).After vaginal birth,the weight of newborn rats and their brain weigh were measured and analyzed.Results Compared with the sham surgery group,the birth weight and brain weight of IUGR group group rats decreased by 24.9% and 17.3%,respectively,the results were significant(Pa

Effect of the pre-hospital systematic treatment on prognosis patients of with severe acute organophosphorus pesticide poisoning / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To investigate if the duration from poisoning to treatment (no treatment period) is related to the prognosis of patients with severe acute organophosphorus pesticide poisoning (SAOPP).</p><p><b>METHODS</b>One hundred and seventy-four patients with the pre-hospital systematic treatment served as the treatment group while 160 patients going to the hospital by themselves without treatment or rejecting gastrolavage served as the control group. Patients in both groups were treated by gastrolavage, pralidoxime chloride, atropine and other expectant treatment. The duration of no treatment period, death, and severe complication were observed. The time of disappearance of symptoms, the recovery time of acetyl cholinesterase (AChE), atropinization time, atropine dosage, pralidoxime chloride dosage, naloxone dosage, hospitalization days and other targets were also observed.</p><p><b>RESULTS</b>The duration of no treatment period in treatment group [(1.2 +/- 0.3) h] was significantly shorter than that in control group [(2.8 +/- 0.5) h, (P < 0.01)]. The mortality rate in treatment group was 6.32% while that in control group 22.5% (P < 0.01). The incidence of respiratory failure, heart injury, brain injury, atropine poisoning, intermediate syndrome, liver injury in treatment group (12.64%, 5.75%, 8.62%, 1.72%, 4.60%, 5.17% respectively) were lower than those in control group (25.63%, 13.75%, 17.50%, 6.25%, 7.50%, 9.38% respectively, P < 0.05 or P < 0.01). The time of symptoms disappearance, the recovery time of AChE, atropinization time, atropine dosage, pralidoxime chloride dosage, naloxone dosage, hospitalization days in treatment group were significantly superior to those in control group (P < 0.05 or P < 0.01).</p><p><b>CONCLUSION</b>The pre-hospital systematic treatment can improve the prognosis of the patients with SAOPP, which is worth popularizing and using.</p>

STUDIES ON THE CHARACTERISTICS OF A MODERATELY THERMOACIDOPHILIC IRON-OXIDIZING BACTERIVM / 微生物学通报

A moderately thermoacidophilic iron-oxidizing bacterium,designated as strain MLY,was isolated from a coal spoil heap in China.The optimum of temperature for growth is 50℃～54℃.The optimum of pH is 1.2～1.4.The strain MLY is facultative autotroph and grows heterotrophically on yeast extract.It is able to oxidize ferrous iron(Fe 2+ ),pyrite(FeS 2),and elemental sulfur(S 0) autotrophically and mixotrophically in the presence of yeast extract.Autotrophic oxidation of elemental sulfur is relative weak.The comparison of ferrous iron and pyrite oxidation between strain MLY and A10 Thiobacillus ferrooxidans,strain indicated that MLY is one time faster than A10.